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. 2020 Aug 20;11(8):665. doi: 10.1038/s41419-020-02893-0

Fig. 6. CASC4 enhances focal adhesions and perturb actin architecture.

Fig. 6

a Immunofluorescence analysis of stable MDA-MB-231 cell lines expressing pIRES-empty vector, CASC4-WT or CASC4-5REL stained for focal adhesion marker (Paxillin; red labeling), phalloidin (F-actin; white labeling), and V5 (green labeling). Yellow arrows are highlighting round actin circles generated in cells expressing CASC4-5REL. b Quantifications of focal adhesions (Paxillin-positive areas) number and size. c Western blot analysis and quantifications of cell lysates from MDA-MB-231 stable cells showing endogenous paxillin and p-paxillin (Y118). d Western blot analysis and quantifications of cells lysates from MDA-MB-231 stable cells incubated with the indicated GST-fusion proteins bound to glutathione beads. The precipitated proteins were detected by immunoblotting with anti- Cdc42 or anti-RhoA antibodies. TCL total cell lysate. These results are representative of at least three independent experiments. Error bars indicate averaged values ± standard error from the mean (SEM). P-values: *P ≤ 0.05 **P < 0.01, n.s. not significant (two-sided Student’s t-test). Scale: 10 µm.