Table 2.
Identity, sources, and working dilution of antibodies used in immunohistochemical studies. Antibodies used that showed reactivity in fish species in past publication.
| Target | Primary antibody supplier | Origin (catalog no) | Dilution | Incubation | Antigen retrieval | Secondary antibody-incubation time |
|---|---|---|---|---|---|---|
| CD34 | Rat Anti-mouse CD34 antibody ( e bioscience, San Diego, CA) |
mouse CD34 Monoclonal Antibody (Clone: RAM34) (Cat.no 14–0.341-85) |
1:100 | Over night |
Boiling in citrate buffer (pH 6.0), 20 min Goat |
Biotinylated goat Anti-Polyvalent, Anti-mouse Igg + Anti-Rabbit Igg, Thermo Fisher Scientific, The UK. Lab Vision Corporation; USA One hour at room temperature |
| CD117 (Kit) | Anti-CD117 (e Bioscience, San Diego, CA) |
Mouse Cd117 Monoclonal Antibody (Clone. ACK2) (Cat. no. 14–1172–82) |
1:100 | Over night | Boiling in citrate buffer (pH 6.0), 20 min | |
| S100 protein | Anti-S100 protein (Dako, Glostrup, Denmark; | Rabbit polyclonal Antibodycode No. Z0311 | 1:100 | Overnight | Boiling in citrate buffer (pH 6.0), 20 min |
Goat anti-rabbit secondary antibody (cat. no. K4003, Envision + TM System Horseradish Peroxidase Labelled Polymer; Dako) 30 min at room temperature |
| Chromogarnin A | Anti Chromogranin A-(Dako A0430) | Polyclonal, Rabbit anti-Human | 1:1000 | One hour at room temperature | boiling in citrate buffer (pH 6.0), 20 min | |
| Desmin | Thermo Fischer scientific (invitrogen) |
Desmin recombinant rabbit monoclonal antibody Clone (sI18-00) Cat.MA5-32,068 |
1:50 | One hourat room temperature | Boiling in citrate buffer (pH 6.0), 20 min |