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. 2019 Apr 18;51(2):239–244. [Article in Chinese] doi: 10.19723/j.issn.1671-167X.2019.02.007

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Annexin V-FITC和PI双标流式细胞仪法检测AM凋亡情况

Detection of AM apoptosis by Annexin V-FITC and PI double-label flow cytometry

A, representative flow cytometric dot plots. X-axis, Annexin-V staining; Y-axis, PI staining. Q1, detection error within the permitted range; Q2, late apoptotic cells and necrotic cells; Q3, early apoptotic cells; Q4, normal living cells (The apoptotic cells are Q2+Q3). B, quantitation of apoptotic cells population as shown in figure A. All the results are expressed as x±s (n=8). *P<0.01 vs. control group; #P<0.05 vs. I/R group.

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