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. 2020 Aug 3;61(10):1. doi: 10.1167/iovs.61.10.1

Figure 1.

Figure 1.

C57BL/6J and rd10 mice present the Rpe65 Leu450Met variant. (A) A schematic representation of the Rpe65 gene and its exons. The forward and reverse primers (forward, 5′-TATGCATACGGACTTGGGTTGA-3′; reverse, 5′-TTCTGGTGCAGTTCCATTCAGT-3) flank the regions of interest, which include codon 450 in the exon 13 (red and blue). The C57BL/6J mice genome, found in DNA databases (GenBank; NC_000069.6), presents the polymorphism (red nucleotide) that confers light-induced damage resistance. (B) For C57BL/6J and rd10 mice, Sanger sequencing results of PCR products after genomic amplification of the region of interest. Boxed region corresponds to the polymorphism. (C) Segment of the RPE65 amino acid sequence highlighting the 450 amino acid (Met) in red.