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. 2020 Aug 3;61(10):1. doi: 10.1167/iovs.61.10.1

Figure 9.

Figure 9.

Effect of environmental light conditions on oxidative stress. (A–H) Representative western blots (A, C) and protein level quantification (B, D, E–H) of 4-HNE, nNOS, RCVRN, and GAPDH in C57BL/6J mice (A, B, E, F) and rd10 mice (C, D, G, H) reared at 5, 50, and 300 lux cyclic light. Mean RCVRN levels in C57BL/6J mice (B) and rd10 mice (D), normalized to GAPDH expression, revealed a light-dependent reduction in rd10 mice. Expression of 4-HNE and nNOS was normalized to RCVRN levels. Expression of both 4-HNE and nNOS exhibited a light-dependent increase in oxidative stress in rd10 mice. (I–J) Images from whole-mounted retinas showing the immunoreactivity of DHE probe at the photoreceptor level in a representative C57BL/6J mouse (I) and a representative rd10 mouse (J) reared at 50 lux cyclic light. Arrowheads point to intracellular DHE-positive cells. (K) Quantification of intracellular DHE-positive cells per mm2 in C57BL/6J and rd10 mice reared at 50 and 300 lux cyclic light. Kruskal–Wallis and Dunn's post hoc test; Mann–Whitney U test. *P < 0.05, ***P < 0.01. Scale bars: 20 µm.