Skip to main content
. 2020 Aug 21;11(8):667. doi: 10.1038/s41419-020-02894-z

Fig. 4. Brd4 facilitates the synthesis of BIRC3 mRNA and eRNAs.

Fig. 4

a, b AGS cells were treated with or without 5 μM JQ1 as indicated for 1 h, followed by H. pylori infection for 1 h. The expression of BIRC3 eRNAs (a) and mRNA (b) was analyzed by qRT-PCR. c AGS cells were treated with or without 5 μM JQ1 for 1 h, followed by H. pylori infection for 2 h. Cell lysates were immunoblotted for the indicated proteins. d, e AGS cells transfected with two different siRNAs against Brd4 were infected with H. pylori for 1 h. Expression of BIRC3 eRNAs (d) and mRNA (e) was analyzed by qRT-PCR. (f) AGS cells transfected with siRNAs against Brd4 as in (d, e) were infected with H. pylori for 2 h. Cell lysates were immunoblotted for indicated proteins. g AGS cells were pre-treated with or without 5 μM JQ1 for 1 h as indicated. Cells were then infected with H. pylori for 1 h followed by treatment with Raptinal (10 µM) for 2 h. Cell lysates were immunoblotted for indicated proteins. h AGS cells transfected with siRNAs against Brd4 as in (d, e) were infected with or without H. pylori for 1 h followed by treatment with Raptinal (10 µM) for 2 h. Cell lysates were immunoblotted for indicated proteins. i AGS cells were infected with or without H. pylori G27 for 1 h. ChIP assays were performed using antibodies against Brd4, RelA, RNAPII and H3K27Ac and probed for the −10.7 k enhancer of BIRC3. All data are shown as the mean ± SD from three independent experiments. *p < 0.05, **p < 0.01, ***p < 0.001.