Figure 1.

Experimental setup. (a) C57BL/6JBomTac (filled circle Taconic Bioscience), C57BL/6JRj (filled triangle Janvier), and C57BL/6J (filled rectangle Charles Rivers), arrived at six to seven weeks of age. (b) After two weeks of acclimatization, a baseline group (n = 6) from each strain was subjected to DEXA scanning and GTT before being sacrificed. Subsequently, 10 weeks diet intervention was initiated on the remaining mice; one group of each strain continued on control chow diet (n = 6), one group changed to a high-fat-diet (HFD) (n = 6) and one group changed to a Western diet (WD) (n = 6) with fructose in the drinking water. At week one and eight average food and water intake was estimated. At week eight GTT was performed. At week two and nine DEXA scanning was performed, and at week nine ITT was performed. Body weight was measured weekly. After 10 weeks of diet intervention mice were sacrificed. (c) Macronutrient content of the chow, HFD and WD diets. During diet intervention some mice succumbed resulting in n < 6 for some treatment groups at the end of the diet intervention.