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. 2020 Aug 20;10:14052. doi: 10.1038/s41598-020-70765-w

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Baseline sacrifice. (a) GTT in C57BL/6JBomTac (filled circle), C57BL/6JRj (filled triangle), and C57BL/6J (filled rectangle) before baseline sacrifice (left) and area under the curve (AUC) (right), n = 6 for each group. (b–d) DEXA scan of baseline groups (n = 6) measuring total tissue mass (TTM), lean % and fat %. (e–j) Sacrifice of the baseline groups (n = 6) was initiated after 4-7 h of fasting (see experimental procedures) and (e) body weight, (f) fasting blood glucose, (c) fasting serum insulin, and weight of (h) liver, (i) eWAT, and (j) iWAT was measured. Data is presented as mean ± SD. Statistical significance is calculated using one-way ANOVA with an n = 6 and p-values are shown in the figure.