TRIM59 expression is upregulated in three retinoblastoma cell lines. Evaluation of mRNA expression using qRT-PCR (A) and protein expression using immunoblotting (B, C) of TRIM59 in the three human retinoblastoma cell lines, Weri-Rb1, Y79, and HXO-Rb44, as well as the normal retinal epithelial cell line ARPE-19. *P < 0.05, **P < 0.01 compared with ARPE-19 cells. Confirmation of knockdown performed by siRNAs targeting TRIM59 in HXO-Rb44 cells at the mRNA(D) and protein (E, F) levels. **P < 0.01 compared with the siNC group. Validation of upregulated mRNA (G) and protein expression (H, I) of TRIM59 in lentivirus transfected Y79 cells. **P < 0.01 compared with vector-transfected cells.