Silencing TRIM59 promotes cell apoptosis. Annexin V-FITC assay showed that increased apoptotic HXO-Rb44 cells (A, B) and decreased apoptotic Y79 (C, D) cells were detected by flow cytometry. *P < 0.05, **P < 0.01 compared with the siNC and vector groups, respectively. Immunoblotting was performed to measure the protein levels of Bcl-2 and cleaved caspase-3 in HXO-Rb44 cells (E, F) and Y79 cells (G, H). **P < 0.01 compared with the siNC and vector groups, respectively.