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. 2020 Aug 5;61(10):9. doi: 10.1167/iovs.61.10.9

Figure 2.

Figure 2.

VHL is downregulated in RB tissues and it is a target gene of miR-222. (A) Normalized expression of VHL in retinal tissues of RB patients (n = 50) and normal retinal tissues (n = 17) detected by RT-qPCR assay. (B) Expression of VHL in retinal tissues of RB patients (n = 50) and normal retinal tissues (n = 17) examined by western blot analysis. (C) Pearson's correlation analysis between VHL and miR-222. (D) Dual-Luciferase Reporter Assays show the binding between miR-222 and VHL. (E) Normalized expression of VHL in transfected Y79 cells by RT-qPCR assay. (F) Protein expression of VHL in transfected Y79 cells measured by western blot analysis. In A and B, *P < 0.05 versus normal retinal tissues. In D, *P < 0.05 versus mimic-NC group. In E and F, *P < 0.05 versus Y79 cells transfected with mimic-NC or inhibitor-NC. Measurement data are expressed as mean ± SD. Data comparisons between two groups were performed by unpaired t-tests. Pearson's correlation coefficient was used to analyze the relationship between miR-222 and VHL. The cell experiment was repeated three times.