Figure 4.

miR-222/VHL/HIF1α axis regulates RB cells resistance to VCR. (A) Normalized expression of miR-222, VHL, and HIF1α in Y79 cells detected by RT-qPCR assay. (B) Expression of VHL and HIF1α in Y79 cells measured by western blot analysis. (C) The IC₅₀ of VCR on Y79 cells in assessed by MTT assay. (D) The cell survival rate of Y79 cells determined by MTT assay. (E) Apoptosis of Y79 cells examined by flow cytometry. ^*P < 0.05 versus oe-NC or inhibitor-NC transfected Y79 cells. Measurement data are expressed as mean ± standard SD. Comparisons among multiple groups were conducted by ANOVA with Tukey's post hoc test. Statistical analysis in relation to time-based measurements within each group was conducted using repeated-measures ANOVA followed by a Bonferroni's post hoc test.