Figure 3.
Thickening of the pia mater in optic nerves from bead-injected eyes. Representative images of optic nerve cross-sections of un-injected eyes of wt and Fbn1Tsk/+mice stained with Weigert's resorcin fuchsin and hematoxylin showing the pia mater stained purple (A). Comparing uninjected eyes, Fbn1Tsk/+ mice had thinner pia mater as compared to wt (B). Analysis (C) comparing nerves from uninjected (left symbols) with those from bead-injected eyes (right symbols) showed significant increase in pia mater thickness in response to bead injection for Fbn1Tsk/+ mice (red symbols), but not for wt (blue symbols). Numbers of animals: 37 wt and 25 Fbn1Tsk/+. Scale bar in A: 20 µm. Dashed lines connect paired eyes (C).