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. 2020 Aug 12;61(10):26. doi: 10.1167/iovs.61.10.26

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Figure 4. — Trehalose induced autophagosome formation in HCECs exposed to hyperosmotic stress. (A) Representative immunofluorescence images of the punctate staining of LC3B in primary HCECs exposed to 450-mOsM hyperosmotic medium with or without prior incubation with 1.0% trehalose. (B) Percentage of autophagic cells. The fields were randomly selected, and at least 100 cells for each sample were analyzed (n = 3). (C) Average puncta per autophagic cell. Three cells were randomly chosen for analysis. (D) Cell viability analyzed by MTT assay. The cell viability was restored in primary HCECs exposed to 450-mOsM hyperosmotic medium with prior incubation with 1.0% trehalose. The data are presented as mean ± SD. ^*P < 0.05, compared within groups.