Table.
Primary and Secondary Antibody Descriptions, Including Targets and Suppliers
| Name (Titer) | Target | Species Raised | Supplier | References* |
|---|---|---|---|---|
| Primary antibodies and stains | ||||
| Anti-TRP2 (1:200) | Melanosome membranes in MB (stages I–II) and melanocytes (stages III–IV) | Goat | Santa Cruz Biotechnology | 14,15,16,17,18 |
| Anti-Iba-1 (1:300) | macrophage/microglia | Rabbit | Wako, 019-19747 | 4,19,20,21,22 |
| Isolectin B4-Biotin (1:100) | Lectin - vascular endothelium and myeloid cells | N/A | Vector Laboratories, B-1205 | 4,21,23 |
| Secondary antibodies and stains | ||||
| Alexa Fluorophore 488 (1:200) | Goat | Donkey | Molecular Probes, A11055 | |
| Alexa Fluorophore 594 (1:400) | Rabbit | Donkey | Molecular Probes, A21207 | |
| Streptavidin-Cy3 (1:400) | Isolectin B4-biotin | N/A | Molecular Probes, 434315 | |
| Hoechst 33342 (1:1000) | Eukaryotic nuclei | N/A | Molecular Probes, H1399 | |
Abbreviations: TRP2 = Tyrosinase related protein 2; Iba-1 = Ionized calcium binding adaptor molecule 1; N/A = not applicable. *Evidence of previous experience with and validation of the respective primary antibodies by the authors.