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. 2020 Aug 20;11(8):666. doi: 10.1038/s41419-020-02898-9

Fig. 1. S5 inhibited macrophage M1-like polarization.

Fig. 1

a Chemical structure of neochromine S5. bd mRNA levels of Il-1β, Il-6, Tnf-α, Inos, Ym1, Fizz1, Cd206, and Arg1 in nonactivated BMDMs (b), M1-BMDMs (c) activated by 10 ng/mL LPS and 10 ng/mL IFN-γ for 6 h, and M2-BMDMs (d) activated by 20 ng/mL IL-4. Cells were treated with 5, 10, and 20 μM of S5 or the same volume of DMSO. Data are means ± SEM of five independent experiments. **P < 0.01, ***P < 0.001 vs. M1 group. e, f Flow cytometry results indicated that CD11c was decreased in M1-BMDMs and CD206 was unchanged in M2-BMDMs. g Cytokines level of IL-1β, IL-6, TNF-α, and IL-10 secreted in M1-BMDMs were assessed by ELISA, and nitrite was assessed by Griess regent. Data are means ± SEM of five independent experiments. *P < 0.05, **P < 0.01, ***P < 0.001 vs. M1 group.