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. 2020 Aug 21;11(8):669. doi: 10.1038/s41419-020-02901-3

Fig. 3. MST Inhibits WBP2-driven Breast Cancer Resulting in Good Prognosis in Xenografts.

Fig. 3

a MST1&2 negatively regulates WBP2 expression in multiple breast cancer cells. A panel of breast cancer cell lines were transiently transfected with luciferase or MST1+2 siRNAs. Forty-eight hours after transfection, WBP2 protein (i) and mRNA (ii) expression was examined by western blot and qPCR. (iii) MST1/2 USF-1 knockdown but not MST1/2 knockdown in MCF7 decreased the WBP2 promoter luciferase reporter activity. b MST1-WT but not kinase-dead K59R mutant significantly abolished the WBP2-driven colony growth of MDA-MB-436 cells. Stable vector- and WBP2-WT-overexpressing MDA-MB-436 cells were transiently transfected with pCI-Neo-SAV and MST1-K59R or MST1-WT for 24h before being seeded for in vitro clonogenic assay and monitored up to 14 days in the presence of hygromycin. (i) Scanned image showing different levels of colony formation in these transfected cells. (ii) Quantitative analysis of the colony formation using the ImageJ software. c MST1-WT but not kinase-dead K59R mutant significantly abolished the WBP2-driven tumor xenograft growth of MDA-MB-436 cells. Stable vector- and WBP2-WT-overexpressing MDA-MB-436 cells were transiently transfected with pCI-Neo-SAV and MST1-K59R or MST1-WT for 24h before being harvested for tumor xenograft study. i The time course line plot of in vivo growth of xenografted tumors. ii Kaplan–Meier survival plot of the xenografted tumor-bearing mice from MST1-K59R-transfected vector or WBP2-WT overexpressing groups. (n =7 mice).