Fig. 5. miR-23a Negatively Regulates WBP2 Protein Expression.

a MST1/2 or Dicer knockdown (i) and overexpression (ii) downregulates and upregulates miR-19a, 19b, and 23a expression respectively in BT549. BT549 cells were transiently transfected with MST1&2 or Dicer siRNAs. Forty-eight hours after transfection, the miRNA expression for miR-19a, 19b, and 23a was examined by qPCR. b The miR-23a, but not miR-19a and -19b mimic and inhibitor downregulates and upregulates the endogenous WBP2 protein expression respectively in HeLa. The HeLa cells were transiently transfected with mimics and inhibitors of miR-19a, 19b, and 23a. Forty-eight hours post-transfection, the total lysates were examined by western blot (i). Expression of miR-19a, 19b, and 23a following transfection of the corresponding mimics (ii) and inhibitors (iii) were validated via qPCR.