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. 2020 Aug 20;10:14038. doi: 10.1038/s41598-020-70932-z

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© This is a U.S. Government work and not under copyright protection in the US; foreign copyright protection may apply 2020, corrected publication 2022

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Predicted Secondary Structure of watermelon ClaPMR2. (A) Open reading frame of ClaPMR2 protein with predicted secondary structure and transmembrane domains using EMBOSS 6.5.7 (Geneious Prime v2019.2.1, https://www.geneious.com/prime/). The ORF consists of the conserved for the N terminal RPW8-EHM targeted motif. The Predicted secondary structure of alpha helix, beta strand, coil and turn are presented in purple cylinders, yellow arrows, grey sinusoids and blue curved arrow. Detailed information of individual sequences is presented in Table S8. (B) Comparative secondary structure and transmembrane domains analysis of Arabidopsis RPW8.1, RPW8.2 with the watermelon ClaPMR2 using EMBOSS 6.5.7 (Geneious Prime v2019.2.1, https://www.geneious.com/prime/). (C) Comparative percentage of sequence (aa) similarity of ClaPMR2/ Cla019831 with AtRPW8.1 and AtRPW8.2 domain. (D) 3D modeling with structural overlap of the RPW8 domain of Arabidopsis thaliana (AtRPW8.1) with the N-terminal domain of watermelon, Cla-RPW8 (USVL531-MDR). Geneious Prime v2019.2.1, https://www.geneious.com/prime/) was used to generate the 3D structure model. ClaPMR2 expression level at 8dpi in USVL531-MDR and USVL677-PMS.