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. 2020 Aug 20;10:14038. doi: 10.1038/s41598-020-70932-z

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© This is a U.S. Government work and not under copyright protection in the US; foreign copyright protection may apply 2020, corrected publication 2022

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SNPs derived CAPS validation on Parent, F₂ and RIL introgressed watermelon lines using DNA Agarose gel. (A) Comparative nucleotide and amino acid consensus sequences with predicted secondary structure alignment of ClaPMR2 encoding protein in Parent (USVL531-MDR & USVL677-PMS), and RIL introgressed watermelon lines (R-201, R-202, R-203, R-204). Black line rectangle area HRH (C-T; Arg-STOP), represents the location of SNP region with substitution position Arg-STOP codon in (ClaPMR2, Citrullus lanatus PM Resistance gene). (B) DNA gel electrophoresis showing the CAPS marker analysis on ClaPMR2 gene in parent lines: USVL531-MDR & USVL677-PMS; RILs: R-201, R-202, R-203 and R-204 and F₂ populations. Since the resistant phenotype is dominant, we observed ClaPMR2 loci, cosegregated with the resistant locus in heterozygous and homozygous individuals with PM resistance lines.