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. 2020 Aug 11;2020:8253904. doi: 10.1155/2020/8253904

Figure 2.

Figure 2

Acupuncture inhibits the expression of TLR4, but not TLR2, in the hippocampus of 2VO rats. (a, b) Representative Western blots and the densitometric analysis of TLR2, TLR4, and its corresponding β-actin bands in protein levels were determined at day 14 after acupuncture treatment using Western blot analysis. The lanes (from left to right) represent the sham, 2VO, 2VO+Acu, and 2VO+Non-acu groups (n = 6). (c, d) The mRNA expressions of TLR2 and TLR4 in the hippocampus were assessed by quantitative real-time PCR at day 14 after acupuncture treatment in the sham, 2VO, 2VO+Acu, 2VO+Non-acu groups (n = 6). (e) Representative photomicrographs of TLR4 immunohistochemistry are shown. (f) Graphic presentations show the positive area of TLR4 in the hippocampal CA1 region at day 14 after acupuncture treatment (n = 6). ∗P < 0.05, ∗∗P < 0.01, and ∗∗∗P < 0.001, compared as indicated.