Skip to main content
. Author manuscript; available in PMC: 2020 Aug 21.
Published in final edited form as: Sci Transl Med. 2019 Aug 21;11(506):eaav4881. doi: 10.1126/scitranslmed.aav4881

Fig. 7. Rapamycin reduces free α-globin in CD34+ cell-derived erythroblasts from individuals with β-thalassemia.

Fig. 7.

CD34+ cells from normal donors (n = 7) or individuals with transfusion-dependent (TD; n = 5) or non-transfusion-dependent (NTD; n = 5) β-thalassemia were grown in culture under conditions that promote erythroid differentiation, treated with rapamycin (10 or 20 μM), MG132 (2.5 μM), or vehicle beginning on day 13 for 2 days, and then analyzed for free α-globin by ion exchange (IE)-HPLC. (A) Representative chromatograms. UV abs, ultraviolet absorbance. (B) Quantification of free α-globin fractions in multiple experiments performed as in (A). Data are mean values ± SDs; ****P < 0.0001; ***P < 0.001; **P < 0.01.