Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2020 Aug 20;19:188. doi: 10.1186/s12944-020-01364-x

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2020

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

PMC Copyright notice

Fig. 3 — Effects of OXT infusion on adipocyte size, lipolysis and adipose tissue macrophage infiltration. (Panel a) Adipocyte size in epididymal tissues of Vehicle (n = 8) or OXT-treated db/db mice (n = 10) and lean control mice (n = 6). (Panel b) Lipolysis of triglycerides in isolated adipocytes from db/db mice incubated with increasing concentration of OXT for 30 min evaluated by release of free fatty acids. Adipocytes isolated from 3 or 4 mice were pooled and assayed in triplicate at each OXT dose. Data represent the mean for each dose from three separate experiments. OXT significantly increased lipolysis in a dose dependent manner; p = 0.003 for OXT dose and * indicates p < 0.01 compared to no oxytocin at all doses by post-hoc analysis

(Panel c). Macrophage infiltration into adipose tissue of Vehicle (n = 8) or OXT-treated db/db mice (n = 10) and lean control mice (n = 6) assessed by immunohistochemistry for F4/80 antigen expression. (Panel d) Densities of crown-like structures in epididymal fat pads of db/db mice treated with vehicle (n = 8) or OXT (n = 9) and lean controls (n = 6) expressed as number per microscopic field (average of 5 fields per mouse tissue). (Panel e). Representative photomicrographs of F4/80 immunostained epididymal fat from vehicle and OXT treated db/db mice and lean control mice, bar = 50 μm. Data were expressed as mean ± SEM for each respective group.