Table 5.
Antigenic, allergenic and physicochemical characteristics of the construct.
| Characteristics | Finding | Remark |
|---|---|---|
| Number of amino acids | 316 | Suitable |
| Molecular weight | 33,614.95 Da | Average |
| Theoretical pI | 8.28 | Slightly basic |
| Chemical formula | C1539H2432N390O439S6 | – |
| Extinction coefficient (at 280 nm in H2O) | 34505 | – |
| Estimated half-life (mammalian reticulocytes, in vitro) | 30 h | – |
| Estimated half-life (yeast-cells, in vivo) | >20 h | – |
| Estimated half-life (E. coli, in vivo) | >10 h | – |
| Instability index of vaccine | 24.33 | Stable |
| Aliphatic index of vaccine | 93.64 | Thermostable |
| Grand average of hydropathicity (GRAVY) | 0.035 | Hydrophobic |
| Antigenicity | 0.6166 | Antigenic |
| Immunogenicity | 1.58298 | Immunogenic |
| Allergenicity | No | Non-allergen |
| Solubility | 0.871753 | Soluble |