Problem	Cause	Solution(s)
Folds appearing on PMP / uneven imaging surface	Most likely mouse hitting the head in the cage to something hard	• Make sure that the PMP is correctly sealed to the bone and a cap is provided to protect the PMP from external environment.
Imaging “gaps” (dark areas in images)	Having bubble or bone under the PMP	• Make sure that all the bubbles and bone dust are washed away before sealing the PMP • If there is any bone growth, PMP could be replaced after removal of the bone
Extensive growth of dural vessels or increased migration of inflammatory cells in the exposure	Extensive manipulation of dura or large gap between PMP and dura	• Inject dexamethasone before surgery • Minimize the manipulation of dura • Minimize the gap between PMP and dura