Table 1:
Recipes
| M9 buffer (1 L) | |
| KH2PO4 | 3 g |
| Na2HPO4 | 6 g |
| NaCl | 5 g |
| 1 M MgSO4 | 1 mL |
| ddH2O | up to 1 L |
| 2x Lysis buffer (5 mL) | |
| HEPES (pH 7.4) | 200 μL |
| 2 M KCl | 250 μL |
| 10% TritonX | 100 μL |
| 1 M MgCl2 | 20 μL |
| 100% glycerol | 1 mL |
| ddH2O | up to 5 mL |
| Add fresh: | |
| 1 M DTT | 20 μL |
| EDTA-free protease inhibitor | 1 tablet |
| phosphatase inhibitor cocktail 2 | 100 μL |
| phosphatase inhibitor cocktail 3 | 100 μL |
| 1x Lysis buffer | |
| Dilute 2x Lysis buffer with an equal volume of ddH20. | |
| 1x Wash buffer 10 mL) | |
| HEPES (pH 7.4) | 300 μL |
| 2 M KCl | 500 μL |
| 10% TritonX | 100 μL |
| 1 M MgCl2 | 20 μL |
| 100% glycerol | 1 mL |
| ddH2O | up to 10 mL |
| 1 M DTT | 20 μL (add fresh) |