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. 2020 Aug 18;8:e9677. doi: 10.7717/peerj.9677

Figure 4. Verification of RNA-Seq Data by quantitative real-time PCR.

Figure 4

(A) Relative normalized expression of 14 selected genes in the normal and aborted pistils using qRT-PCR. Error bars indicate the standard errors. AUX-IAA (auxin-responsive protein IAA), ARF (auxin response factor), TF (PIF4, phytochrome-interacting factor 4), ACO (1-aminocyclopropane-1-carboxylate oxidation), ETR (ethylene receptor gene), ERDBF3 (ethyleneresponse DNA binding factor 3), ERTF10 (ethylene responsive transcription factor 10), AP2 (like ethylene-responsive transcription factor), TCH4 (xyloglucan endotransglucosylase/hydrolase), CYCD3 (cyclin D3), JAR1 (jasmonic acid-amino synthetase), NPR1 (BTB/POZ domain and ankyrin repeat-containing protein NOOT2), TGA (TGACG-sequence-specific DNA-binding protein), PAL (phenylalanine ammonia-lyase). (B) Comparison of the expression ratios of 14 selected genes in using RNA-seq and qRT-PCR.