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. 2020 Aug 21;2(4):226–231. doi: 10.1016/j.bsheal.2020.08.004

Fig. 1.

Fig. 1

Identification of SARS-CoV-2 S protein expression and SARS-CoV-2 pseudotyped virus.

(A) Construction and identification of S expressing plasmid. SARS-CoV-2 S protein gene was inserted in the pCDNA3.1 vector. (B) Immunofluorescence assay for S protein expression in pcDNA3.1-SARS-CoV-2 S plasmid. The expression was determined using mouse pAb against SARS-CoV-2 S protein and convalescent serum samples from COVID-19 patients. (C) Identification of S protein expression in SARS-CoV-2 pseudotyped virus by immunoblot assay. Bands corresponding to SARS-CoV-2 S and HIV-1 p24 proteins were detected at the same sample line in the gel.