Table 1.
Some characteristics of nucleoproteins and phosphoproteins of MNV involved in condensates formation.
| Protein (UniProt identifier) | Sequence | Asp/Glu frequencya | Lys/Arg frequencyb | Net charge per residuec | Phe/Tyr/Trp frequencyd |
|---|---|---|---|---|---|
| RABV P (P22363) | Full length 1–297 |
20/29 16.5% |
20/17 12.5% |
−0.040 | 11/6/3 6.7% |
| IDD1 59–90 |
5/6 35.5% |
2/2 12.9% |
−0.218 | 1/1/0 6.5% |
|
| IDD2 132–182 |
1/4 9.8% |
4/5 17.6% |
+0.078 | 2/0/0 3.9% |
|
| MeV P (P03422) | Full length 1–507 |
32/41 14.4% |
31/27 11.4% |
−0.029 | 7/7/2 3.2% |
| P tail 38–304 |
16/24 15% |
8/14 8.3% |
−0.068 | 3/4/1 3.0% |
|
| P loop 376–449 |
5/3 10.8% |
8/5 17.6% |
+0.068 | 1/0/0 1.35% |
|
| EBOV VP35 (Q05127) | Full length 1–340 |
17/20 10.9% |
16/18 10% |
−0.009 | 9/6/3 5.3% |
| IDD1 48–81 |
1/1 5.9% |
2/1 8.8% |
+0,029 | 0/1/0 2.9% |
|
| IDD2 159–213 |
1/9 18.2% |
1/3 7.3% |
−0,109 | 1/2/1 7,3% |
|
| RABV N (P16285) | Full length 1–450 |
22/32 12% |
26/23 10.9% |
−0,011 | 27/21/3 11.3% |
| MeV N (Q89933) | Full length 1–525 |
37/36 13.9% |
17/42 11.2% |
−0.112 | 17/12/5 6.5% |
| Ntail 392–525 |
13/11 17.9% |
3/15 13.43% |
−0,045 | 1/2/0 2.2% |
|
| EBOV NP (P18272) | Full length 1–739 |
59/59 16% |
38/33 9.6% |
−0.063 | 26/21/4 6.9% |
| NTail 413–640 |
41/16 25% |
7/11 7.9% |
−0.171 | 2/5/0 3% |
Both full length and IDD sequences have been analyzed.
RABV N do not contain IDDs. Putative IDDs for EBOV NP and VP35 have been identified using IUPred2A [151].
a
Number of Asp residues and number of Glu residues. Frequency of negatively charged residues.
b
Number of Lys residues and number of Arg residues. Frequency of positively charged residues.
c
Net charge per residue = (number of Arg residues + number of Lys residues − number of Asp residues − number of Glu residues) / total number of residues in the sequence.
d
Number of Phe residues, number of Tyr residues and number of Trp residues. Frequency of aromatic residues.