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. Author manuscript; available in PMC: 2020 Aug 21.
Published in final edited form as: FASEB J. 2020 Mar 30;34(5):6741–6756. doi: 10.1096/fj.201902838R

Figure 6.

Figure 6.

IFT80 ablation impairs cillogenesis and decreases collagen expression in the mouse IVD.

(A) Immunofluorescence staining for acetylated tubulin, Col2α1 and DAPI showing the length of primary cilia and Col2α1 expression in the GP, EP, IAF and NP from IFT80fl/fl and IFT80fl/fl; Col2-creERT mice. Red: Acetylated tubulin, Green: Col2α1 and Blue: DAPI. (B) Quantitative measurements of the percentage of the cells with primary cilia to the total cells per view of (A) (n=6 mice per group). (C) Immunofluorescence staining for acetylated tubulin, Col1α1 and DAPI showing the primary cilia and Col1α1 expression of the outer AF (OAF) from IFT80fl/fl and IFT80fl/fl; Col1-creERT mice. Red: Acetylated tubulin, Green: Col1α1 and Blue: DAPI. (D) Quantitative measurements of the percentage of the cells with primary cilia to the total cells per view (C) (n=6 mice per group). White arrow: cilia were detected by acetylated tubulin antibody. All data are reported as the mean ± s.d. Statistical significance was determined by Student’s t-test. *P<0.05, **P<0.01, ***P<0.0001. NS = not statistically significant.