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. 2019 Sep 30;23(1):53–65. doi: 10.1093/ijnp/pyz051

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© The Author(s) 2019. Published by Oxford University Press on behalf of CINP.

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited. For commercial re-use, please contact journals.permissions@oup.com

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Figure 5. — Behavioral phenotype of conditional ablation MCH neurons. (A) MCH immunoreactivity in the lateral hypothalamus of iDTR⁺/Cre⁻ and iDTR⁺/Cre⁺ mice following DT administration. (B) Counts of MCH immunoreactive neurons in DT-treated iDTR⁺/Cre^- and iDTR⁺/Cre⁺ mice. Unpaired t test (male: t = 13.67, P < .0001, female: t = 10.72, P < .0001), ***P < .0001. Data are presented as mean ± SEM. (C) Distance mice travelled in 60 minutes of locomotion test, unpaired student t test: male: t = 0.589, P = .567 (n = 6); female: t = 0.001, P = .99 (n = 8). Data are presented as mean ± SEM. (D) Number of marbles buried in 30 minutes: male: t = 2.27, P = .046 (n = 6), female: t = 0.3, P = .76. Unpaired student t test iDTR+/Cre- vs iDTR⁺/Cre⁺, *P < .05 (n = 8). (E) Time mice spent interacting with empty cup and control mice in social interaction test: male: genotype effect, 2-way ANOVA, F_1,36 = 0.039, P = .84. Bonferroni post hoc test: empty cup vs unfamiliar mouse, iDTR⁺/Cre⁻: t = 5.945, P < .001, iDTR⁺/Cre⁺: t = 5.553, P < .001 (n = 10); female: genotype effect, 2-way ANOVA, F_1,36 = 0.5725, P = .45. Bonferroni post hoc test: empty cup vs unfamiliar mouse, iDTR⁺/Cre⁻: t = 5.702, P < .001, iDTR⁺/Cre⁺: t = 3.634, P < .001. **P < .01, ***P < .001 (n = 12 and 8 for iDTR⁺/Cre⁻ and iDTR⁺/Cre⁺, respectively). Data are presented as mean ± SEM. (F) Prepulse inhibition ratio: male: 2-way ANOVA, genotype effect (F_1,54 = 15.22, P = .0003), prepulse intensity effect (F_2,54 = 9.049, P = .0004) followed by Bonferroni post hoc test: iDTR⁺/Cre⁻ vs iDTR⁺/Cre⁺, *P < .05 (n = 7); female, 2-way ANOVA, genotype effect (F_1,39 = 14.68, P = .0005), prepulse intensity effect (F_2,39 = 11.23, P < .0001). Bonferroni post hoc test: iDTR⁺/Cre⁻ vs iDTR⁺/Cre⁺, *P < .05 (n = 8 and 7 for iDTR⁺/Cre⁻ and iDTR⁺/Cre⁺, respectively).