Key resources table.
| Reagent type (species) or resource |
Designation | Source or reference | Identifiers | Additional information |
|---|---|---|---|---|
| Antibody | Rabbit polyclonal anti-caspase-3 | Sigma-Aldrich | C8487 RRID:AB_476884 |
IF (1:500) IHC-Fr (1:500) |
| Antibody | Rabbit polyclonal anti-Ki-67 | Novus Biologicals | NB500-170 RRID:AB_10001977 |
IF (1:300) IHC-Fr (1:500) IHC-P (1:2000) |
| Antibody | Mouse monoclonal Anti- p16Ink4a | Abcam | ab54210 RRID:AB_2059963 |
IHC-P (1:500) |
| Antibody | Polyclonal Alexa Fluor 488 (Donkey anti-Rabbit) | Invitrogen Thermo Fisher | A-21202 RRID:AB_141607 | IF (1:1000) |
| Antibody | Polyclonal Alexa Fluor 555 (Donkey anti-Mouse) | Invitrogen Thermo Fisher | A-31570 RRID:AB_2536180 | IF (1:1000) |
| Antibody Array | Human Cytokine Antibody Array C5 | RayBiotech, Inc | AAH-CYT-5–2 RRID:AB_10185250 | |
| Chemical compound, drug | o-Vanillin | Sigma-Aldrich | 120804 | |
| Chemical compound, drug | RG-7112 | Selleck Chemicals | S7030 | |
| Chemical compound, drug | DMSO | Sigma-Aldrich | D8418 | |
| Commercial Kit | Apoptosis detection | Abcam | ab176749 RRID:SCR_018379 | |
| Commercial Kit | Caspase 3/7 activity | AAT Bioquest 13503 | Amplite Fluorimetric Catalog # 13503 | |
| Commercial Kit | qScript cDNA Synthesis Kit | Quanta Biosciences | 95047–500 | |
| Commercial Kit | Goat anti-Mouse and Rabbit specific HRP/DAB Detection IHC Kit | Abcam | ab64264 | |
| Commercial Kit | Mouse monoclonal Anti- p16Ink4a | Roche, Ventana Laboratories | CINtec Plus (9531) RRID:SCR_018380 | Prediluted |
| Commercial Kit | Mycoplasma PCR Detection Kit | ZmTech Scientific | M209001 | |
| Commercial Assay | Custom Taqman (R) Gene Expression 96-Well Fast Plates | Thermo Fisher | 4413255 | See Supplementary file 1 for each gene product ID |
| Commercial Assay | Luminex immunoassay 19-PLEX plate - Human Cytokine PROCARTAPLEX | Life Technologies | MXMFXHX | |
| Software | ImageJ | ImageJ http://imagej.nih.gov/ij/ | RRID:SCR_003070 | |
| Software | GraphPad Prism 8 | GraphPad Prism https://www.graphpad.com | RRID:SCR_015807 | |
| Software | Ingenuity Pathway Analysis (IPA) | Ingenuity Systems | RRID:SCR_008653 | |
| Software | R package ggplot2 | Wickham, 2016 | ISBN 978-3-319-24277-4 | |
| Software | MIPAV | NIH Center for Information Technology | RRID:SCR_007371 | |
| Software | Image Quant TL array analysis | GE Healthcare | RRID:SCR_018374 | |
| Software | FlowCytomix Pro2.2.1 | eBioscience | RRID:SCR_018375 | |
| Software | AxioVision LE64 | Zeiss | RRID:SCR_002677 | |
| Other | DMMB | Sigma-Aldrich | 341088 | |
| Other | Alamar Blue reagent | Thermo Fisher | DAL1025 | |
| Other | DAPI stain | Invitrogen | D1306 | (1 µg/mL) |
| Other | Safranin-O | Sigma-Aldrich | S2255 | |
| Other | TaqMan Fast Universal PCR Master Mix (2×) | Thermo Fisher | 4366073 | |
| Cell line | Primary Human Nucleus Pulposus (NP) and Annulus Fibrosis (AF) | Primary Cells | Human Biological samples. See Table 1 for demographics. | Cell line maintained in Haglund Lab. |