Table 1. Cryo-EM data collection and refinement statistics.
| hFzd5ICL3BRIL/anti-BRIL Fab/anti-Fab Nb (EMD-21927) (PDB ID: 6WW2) |
|
|---|---|
| Data collection and processing | |
| Magnification | 81,000x |
| Voltage (kV) | 300 |
| Electron exposure (e/Å2) | 50 |
| Defocus range (μm) | −0.8 to −2.0 |
| Pixel size (Å) | 1.078 |
| Symmetry imposed | C1 |
| Initial particle images (no.) | 6,483,398 |
| Final particle images (no.) | 369,704 |
| Map resolution (Å) FSC threshold |
3.70 0.143 |
| Map resolution range (Å) | 3.2 to 5.9 |
| Refinement | |
| Initial model used (PDB code) | 5L7D, 6BD4, 6ANI, 6CBV |
| Model resolution (Å) FSC threshold |
3.7/4.1 0.143/0.5 |
| Map sharpening B factor (Å2) | −124.4 |
| Model composition Non-hydrogen atoms Protein residues |
7,594 982 |
|
B factors (Å2) Protein |
89.59 |
| R.m.s. deviations Bond lengths (Å) Bond angles (°) |
0.010 1.083 |
| Validation MolProbity score Clashscore Poor rotamers (%) |
1.87 5.73 0 |
| Ramachandran plot Favored (%) Allowed (%) Disallowed (%) |
89.59 10.31 0.10 |