A-I) C57BL/6 mice were inoculated with GL261 cells and treated as in
Figure 5, and tumors were isolated on day 23 and analyzed by flow cytometry (n =
5 per group). Shown are A) CD8α+ T cells among all T cells, B) PD-1
receptor expression on CD8α+ T cells, C) CD25+Foxp3+ regulatory T cells
among CD4+ T cells, D) ratio of CD8α+ T cells to regulatory T cells, and
E) the ratio of M1 (CD206- F4/80+) to M2 (CD206+ F4/80+) macrophages.