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. 2020 Aug 21;6:31. doi: 10.1038/s41522-020-00141-z

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 2 — Percentage fluorescent planktonic and adhering staphylococci due to uptake of fluorescent calcein as a function of adhesion force. Lines indicate an exponential fit used to derive plateau levels and critical adhesion forces. Percentage fluorescent staphylococci were calculated with respect to the total number of planktonic bacteria or bacteria adhering to each substratum surface. Dashed lines indicate the 95% confidence band. Horizontal error bars represent standard deviations over at least 45 force–distance curves, comprising for one probe three different spots recording five force–distance curves in each spot. Three probes prepared from three separate bacterial cultures. Vertical error bars represent standard deviations over nine different fluorescence images obtained from three different bacterial cultures.