Table 3.
Bacterial state | Exposure to | S. aureus RN4220 % fluorescent staphylococci | S. aureus RN4220 ΔmscL % fluorescent staphylococci |
---|---|---|---|
Planktonic | Calcein → Formaldehyde | 3 ± 4 | 7 ± 7 |
Planktonic | Formaldehyde → Calcein | 9 ± 8 | 13 ± 4 |
Adhering | Calcein → Formaldehyde | 45 ± 11 | 45 ± 11 |
Adhering | Formaldehyde → Calcein | 6 ± 3 | 9 ± 5 |
Adhering | Gadolinium → Calcein | 9 ± 3 | 6 ± 2 |
Fluorescence of planktonic and staphylococci adhering to polystyrene wells due to uptake of calcein prior to and after exposure of bacteria to formaldehyde33 or gadolinium to induce channel closure34,37–39,50. ± signs represent standard deviations over nine different fluorescence images obtained from three different bacterial cultures.