Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2020 Aug 21;10:14084. doi: 10.1038/s41598-020-71100-z

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2020

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

TGF-β stimulation does not induce the loss of adipocyte marker expression under standard culture conditions in primary mouse adipocytes differentiated ex vivo. (a) Schematic of the transgenic mouse model used. (b) Experiment outline to test the effect of TGF-β on primary adipocytes using immunofluorescent detection of GFP and adipocyte markers PPARγ and C/EBPα. Primary SVF cells from Adipoq:Cre nT/nG mice were expanded and differentiated into adipocytes in vitro. TGF-β was added to the culture media at the end of differentiation (day 0) and cells were analyzed at days 0, 2, 4 and 6 using immunofluorescent staining. (c) Representative fluorescent images of staining against PPARγ at day 6 after adding stimulus. GFP expression is colocalized with PPARγ expression in the nuclei of both control and TGF-β-treated cells. Scale bar: 50 µm. (d) Percentage of GFP-positive cells expressing adipocyte markers PPARγ and C/EBPα. Two-tailed Student t tests with Benjamini–Hochberg correction; FDR = 0.01; n = 3–8 technical replicates, all time points p > 0.05.