Figure 2.

The mCitrine-PPARG cell line can be used to track the endogenous expression of the adipogenic marker PPARγ2 at the single-cell level. (a) Schematic and representative fluorescent images of the mCitrine-PPARG OP9 cell line which also includes a fluorescent nuclear marker H2B-mTurquoise (CFP). Scale bar: 100 um. (b) Distribution of single-cell mCitrine-PPARγ expression at the end of differentiation protocol (day 4). Cells were binned based on the nuclear mCitrine signal and the bins were color-coded. (c) Time course analysis of median mCitrine expression in six bins depending on initial mCitrine expression in differentiated non-replated cells. Data for untreated control and cells treated with 2 ng/ml TGF-β added at 2 h are shown.