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. 2020 Aug 21;10:14070. doi: 10.1038/s41598-020-70987-y

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© The Author(s) 2020

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Histological quantification of D2.mdx skeletal muscle fibrosis. Representative picrosirius red and hematoxylin and eosin (H&E) images and fibrosis quantifications for (a,b) diaphragm and (c,d) gastrocnemius muscles from male wild-type (4 month-old DBA/2J) and 4, 6, and 12 month-old D2.mdx mice (n = 6–15). Values are reported as mean ± SEM, and data were analyzed using one-way ANOVA followed by Tukey post-hoc tests (*p ≤ 0.05 vs. wild-type levels; ^#p ≤ 0.05 vs. 4- and 6-month levels).