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. 2020 Aug 21;11(8):673. doi: 10.1038/s41419-020-02800-7

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 1 — a Total number of H3WT and H3.3K27M-mut gliomaspheres after 5 days. b Quantification and (c) bright-field images of H3WT and H3.3K27M-mut gliomasphere formation after irradiation with 8 Gy (after 24 h) after 5 days. Spheres where scored according to their size: large (>0.1 mm) and small (<0.1 mm), # large and Δ small spheres, scale bar 1 mm. d, e Cell viability assessed by MTT assay, reflecting the cell growth over time of primary H3.3K27M-mut-DIPG and H3WT-pedHGG/DIPG cells grown under stemness- (gliomaspheres) and differentiation- (monolayer) conditions. f Protein expression of stemness-associated markers Oct4, Nestin, and Sox2 in cytoplasmatic and nuclear protein fractions of H3WT and H3.3K27M gliomaspheres, assessed by western blotting, β-actin served as loading control.