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. 2020 Aug 21;11(8):673. doi: 10.1038/s41419-020-02800-7

Fig. 3. Combined treatment with JQ1 and ICG-001 results in strong cytotoxic effects and significantly suppresses tumor-associated characteristics of H3K27M-mut-DIPG cells.

Fig. 3

a Colony-formation assay (*p < 0.05) and (b) sphere-formation assay showing the total number of gliomaspheres, scored according to their size: large (>0.1 mm), mid-sized (0.1–0.3 mm), and small (0.05–0.1 mm), p < 0.05 for * large, # midsize, and Δ small spheres. c Migration and (d) invasion of H3K27M-mut-DIPG cells after treatment with ICG-001 and JQ1, as indicated. (e–g) MTT cell viability assays of H3K27M-mut-DIPG cells grown under (f) stemness- (gliomaspheres) and (g) differentiation- (monolayer) conditions after ICG-001 and JQ1 treatment (at days 1 and 3) in combination with 8 Gy irradiation (at day 2) after 6 days of total treatment, (e) as schematically shown (DMSO control, 0 Gy = 100%, *p < 0.05 with respect to similarly treated, nonirradiated cells).