Fig. 6. Inadvertently activated gene expression in DIPG-007 cells by single treatment with ICG-001 and JQ1 is reversed by combined treatment due to inhibition of associated super enhancers.

a–c qPCR validating the gene expression of (a) ALDH1A3, (b) KRT80, and (c) RREB1 in DIPG-007 and GBM-001 cells after 48 h of treatment with ICG-001 and JQ1, as indicated. P < 0.05. d–f Occupancy profiles of H3K27ac at ALDH1A3, KRT80, and RREB1 in cells treated with JQ1 or ICG-001 overlaid on DMSO-treated cells for comparison. Super enhancers in control cells are shown in gray. g Graphical model depicting the mutual effect of JQ1 and ICG-001 on detrimental programs affecting each other and leading to an increased effect of combinatorial therapy.