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. 2020 Aug 10;11(8):696. doi: 10.1038/s41419-020-02783-5

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 1 — a Exosomes were extracted from mesenchymal stem cell (hMSC) supernatants under normal and hypoxic conditions. b Surface markers of hMSCs detected by flow cytometry: positive for CD44, CD105, and CD73; negative for CD31, CD34, and CD45. c Representative image showing the morphology of normoxic hypoxic hMSCs under light microscopy. d Electron micrograph-analyzed hMSC-derived exosomes. The red arrow indicates exosomes. Scale bar: 100 nm. e Western blot showing the protein level of TSG101, CD63, and CD81 in normoxia-conditioned hMSC-derived Exo (Nor-Exo) and hypoxia-conditioned hMSC-derived Exo (Hypo-Exo), respectively. f Size distribution of Nor-Exo and Hypo-Exo determined by nanoparticle tracking analysis (NTA). X axis represents size of exosomes. Y axis represents the particle concentration in 1 ml PBS (before dilution). g H9c2 cardiomyocytes were cultured in the presence or absence (control) of Dil-labeled exosomes (red) at 37 °C for 6 and 24 h. The nucleus was stained with DAPI (blue). The white arrow indicates Dil-labeled exosomes absorbed by exosomes.