Figure 3.

Flowchart of follow-up analyses, including conditional analysis and single variant test to identify driving rare variants. For conditional analysis, significant results were defined as p-value < 5×10⁻⁵ in meta-analysis of discovery studies, and p-value < 0.05/10 (Bonferroni correction for 10 gene-lipid pairs with p-value < 5×10⁻⁵ in discovery phase) in meta-analysis of replication studies. For single variant test to identify driving rare variants, we included variants with minor allele count at least 5 and present in at least 2 studies. Bonferroni correction for number of SNPs tested in discovery phase and number of SNPs taken forward to replication were applied separately for joint test and interaction test for each lipid trait.