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. Author manuscript; available in PMC: 2020 Dec 18.
Published in final edited form as: Nature. 2020 Jun 18;584(7821):437–442. doi: 10.1038/s41586-020-2456-9

Extended Data Figure 4. Diagrammatic representation of the SARS-CoV-2 pseudovirus luciferase assay.

Extended Data Figure 4.

a, Co-transfection of pNL4-3ΔEnv-nanoluc and pSARS-CoV-2 spike vectors into 293T cells (ATCC) leads to production of SARS-CoV-2 Spike-pseudotyped HIV-1 particles (SARS-CoV-2 pseudovirus) carrying the Nanoluc gene. b, SARS-CoV-2 pseudovirus is incubated for 1 h at 37°C with plasma or monoclonal antibody dilutions. The virus-antibody mixture is used to infect ACE2-expressing 293T cells, which will express nanoluc Luciferase upon infection. c, Relative luminescence units (RLU) reads from lysates of ACE2-expressing 293T cells infected with increasing amounts of SARS-CoV-2 pseudovirus. Error bars represent standard deviation of triplicates, two experiments.