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. Author manuscript; available in PMC: 2021 Jan 16.
Published in final edited form as: Oncogene. 2020 Jul 16;39(34):5649–5662. doi: 10.1038/s41388-020-01391-z

Figure 2: ER+ breast cancer cells show increased reliance on OXPHOS versus surrounding bone marrow stromal cells.

Figure 2:

A. Representative images of NADH lifetimes in co-culture spheroids. Red and purple on the pseudocolor scale indicate longer and shorter NADH lifetimes, respectively. A longer NADH lifetime represents increased usage of cellular NADH for OXPHOS over glycolysis and a shorter NADH lifetime represents a preferential use of NADH for glycolysis over OXPHOS. White boxes show cancer cells marked with mCherry NLS (pseudocolored grey in presented images). Scale bar = 30 μm, B. Box and whisker plots show distributions of NADH lifetimes for cancer (black) and stromal (grey) cells in mono and co-culture spheroids. Lines represent a significant (**** = p < 0.0001) difference in NADH lifetime. N = 26–67 images over 5–10 spheroids, C. Box and whisker plots show distributions of NADH lifetimes for stromal cells non-adjacent (magenta) and adjacent (green) to cancer cells. Cell type of interest is bolded in x-axis label. There were no significant differences between non-adjacent and adjacent cells at p = 0.05. N = 26–67 images over 5–10 spheroids