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. Author manuscript; available in PMC: 2021 Jan 16.
Published in final edited form as: Oncogene. 2020 Jul 16;39(34):5649–5662. doi: 10.1038/s41388-020-01391-z

Figure 3: ER+ breast cancer cells activate Akt to a greater extent than ERK in co-culture spheroids with bone marrow stromal cells.

Figure 3:

A. Representative images show breast cancer cells (defined by mCherry-H2B fusion) expressing both ERK and Akt KTRs grown in spheroids with stromal cells. Regions of interest define cancer cell nuclei in all images. Cell type of interest is bolded in image label. Scale bar = 15 μm, B. Box and whisker plots show quantified activities of ERK and Akt KTRs presented as the log base 2 of the ratios of cytoplasmic to nuclear fluorescence intensities (CNR) for each KTR in single cells. A higher ratio defines greater kinase activation. Cell type of interest is bolded in x-axis label. Lines show pair-wise comparisons with * = p < 0.05 or **** = p < 0.0001. N = 125–420 cells from 8 spheroids.