Figure 3. CS-, CR-Related Activity, and Selectivity of PL Neurons.

(A–C) Three different PL neurons. Top: rasters, where ticks represent individual spike times and each row represents a trial. Bottom: Z-scored peri-event histograms (PEHs) of firing rates. In (A1)–(C1), rasters and PEHs are referenced to CS onset, whereas in (A2)–(C2), they are referenced to CR onset. (A) Cell with a CS response but no CR correlate. (B) Cell with no CS response that fires in relation to the CR. (C) Cell with both, a CS response and a CR correlate.

(D) Comparison between Z-scored averaged firing rate ± SEM of PL cells during AA (black) versus no avoidance (green), referenced to CS onset. Only cells increasing their firing rates during AA are included in this panel.

(E) Comparison between Z-scored average firing rate ± SEM of PL cells during correct (black) and error (red) CS-S trials. The number of cells included in this analysis is smaller than in (D) because error trials did not occur in all recording sessions. This figure is based on ten error trials from seven recording sessions.

(F) Comparison between Z-scored average firing rate ± SEM of PL cells during correct (black) and error (red) CS-R trials (60 error trials from 12 sessions).

(G1) Comparison between the incidence of cells with excitatory (red) or inhibitory (blue) responses to only one CS-R (top) or CS-S (bottom) among LA (left), BL (middle), and PL (right) neurons. Percentages represent selective cells out of total number of responsive cells. The total numbers of CS-R excited cells were 24 in LA, 22 in BL, and 40 in PL. The total numbers of CS-S excited cells were 31 in LA, 26 in BL, and 47 in PL. The total numbers of CS-R inhibited cells were 25 in LA, 52 in BL, and 49 in PL. The total numbers of CS-S inhibited cells were 20 in LA, 46 in BL, and 38 in PL.

(G2) Example of PL cell with inhibitory response to CS-R2 but not to CS-R1. Comparison between the incidence of cells with excitatory (red) or inhibitory (blue) CR-related activity in relation to only one CS-R (top) or one CS-S (bottom). The total numbers of RA-excited cells were 28 in LA, 27 in BL, and 60 in PL. The total numbers of AA-excited cells were 47 in LA, 37 in BL, and 68 in PL. The total numbers of RA-inhibited cells was 35 in LA, 44 in BL, and 148 in PL. The total numbers of AA-inhibited cells were 47 in LA, 37 in BL, and 68 in PL.

(H1) Comparison between the incidence of cells with excitatory (red) or inhibitory (blue) responses to only one RA (top) or AA (bottom) among LA (left), BL (middle), and PL (right) neurons. Percentages represent selective cells out of total number of responsive cells. The total numbers of RA excited cells were 28 in LA, 27 in BL, and 60 in PL. The total numbers of AA excited cells were 47 in LA, 37 in BL, and 68 in PL. The total numbers of RA inhibited cells were 35 in LA, 58 in BL, and 94 in PL. The total numbers of AA inhibited cells were 45 in LA, 44 in BL, and 148 in PL.

(H2) Example of PL cell activated in relation to AA elicited by CS-S1 but not CS-S3.

(G2 and H2) Top: rasters, where ticks represent individual spike times and each row represents a trial. Bottom: Z-scored PEHs of firing rates.