Figure 2.

Measurements using recombinant TwinStrep-HDAC10 fusion protein alone (black), with DMSO (red), Tubastatin A (green), and oxa-Tubastatin A (blue). Protein was tested at 3 μM and inhibitors at 6 μM, giving a protein:inhibitor ratio of 1:2. For the two control experiments (black and red), n = 1. For the inhibitor experiments (blue and green), n = 2; the data shown represent the mean of the two experiments.