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. Author manuscript; available in PMC: 2021 Jan 13.
Published in final edited form as: Nat Immunol. 2020 Jul 13;21(9):1022–1033. doi: 10.1038/s41590-020-0725-2

Extended Data Fig. 8. Antioxidants reverse endogenous tumor-associated T cell dysfunction.

Extended Data Fig. 8

(a) Production of IFN-γ and TNF following re-stimulation with PMA and ionomycin in chronically stimulated T cells with or without N-AC supplementation under normoxic or hypoxic conditions. Experiment was repeated two times with similar results. (b-c) Oxygen consumption rate (OCR) of OT-I T cells chronically co-cultured with B16 melanoma cells with or without anti-PD-L1 antibodies and with or without N-AC supplementation at baseline or in the presence of ATP synthase inhibition (Oligo), uncoupling agents (FCCP), or complex III/IV inhibition (Rot/AA). (d) Production of IFN-γ and TNF following re-stimulation with PMA and ionomycin in chronically stimulated T cells with or without MitoTEMPO or Trolox supplementation as indicated. Experiment was repeated two times with similar results. P values were calculated by unpaired, two-sided Student’s t-test (c). Data are presented as the mean ± s.d. of n=4 biologically independent samples from a representative experiment (b-c). *P<0.05.